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Official Description

Biopsy, soft tissue of thigh or knee area; deep (subfascial or intramuscular)

© Copyright 2025 American Medical Association. All rights reserved.

Common Language Description

The CPT® Code 27324 refers to a biopsy procedure performed on the soft tissues of the thigh or knee area, specifically targeting deep tissues that are either subfascial or intramuscular. Soft tissues encompass a variety of structures, including muscles, tendons, fat, blood vessels, lymph vessels, nerves, and the tissues that surround joints. This procedure is typically conducted under local, regional, or general anesthesia, or conscious sedation, depending on the specific site and depth of the biopsy being performed. Prior to the biopsy, the skin over the designated area is thoroughly cleansed to minimize the risk of infection. A surgical incision is then made, allowing the physician to carefully dissect through the tissue layers down to the mass or lesion of interest, while taking precautions to avoid damaging any nearby blood vessels and nerves. Once the tissue sample is obtained, it is sent to a laboratory for histological evaluation, which is reported separately. After the sample collection, the incision is closed using sutures. It is important to note that this code is specifically for deeper biopsies that require more extensive dissection compared to a superficial biopsy, which is coded under 27323.

© Copyright 2025 Coding Ahead. All rights reserved.

1. Indications

The biopsy procedure coded as CPT® 27324 is indicated for various clinical scenarios where a deeper tissue sample is necessary for diagnostic purposes. The following conditions may warrant this procedure:

  • Suspicious Mass or Lesion A palpable or imaging-detected mass in the thigh or knee area that requires histological evaluation to determine its nature, such as benign or malignant characteristics.
  • Unexplained Pain or Swelling Persistent pain or swelling in the thigh or knee region that does not respond to conservative treatment and necessitates further investigation to identify underlying pathology.
  • Infectious Process Suspected infections involving deeper soft tissues that may require tissue sampling to identify the causative organism and guide appropriate treatment.
  • Inflammatory Conditions Conditions such as myositis or other inflammatory disorders affecting the muscles or surrounding tissues that require confirmation through histological examination.

2. Procedure

The procedure for a deep biopsy of the thigh or knee area, as described by CPT® 27324, involves several critical steps to ensure accurate tissue sampling and patient safety. The following outlines the procedural steps:

  • Preparation The patient is positioned appropriately, and the area of the biopsy is marked. Anesthesia is administered based on the depth and location of the biopsy, which may include local, regional, or general anesthesia, or conscious sedation.
  • Skin Cleansing The skin over the biopsy site is thoroughly cleansed with an antiseptic solution to reduce the risk of infection during the procedure.
  • Incision A surgical incision is made over the biopsy site, allowing access to the underlying tissues. The incision is carefully planned to provide adequate exposure while minimizing trauma to surrounding structures.
  • Tissue Dissection The surgeon dissects through the layers of tissue down to the mass or lesion. This step requires careful handling to protect vital structures such as blood vessels and nerves that may be present in the area.
  • Tissue Sample Collection Once the lesion is reached, a representative tissue sample is obtained. This sample is critical for subsequent histological evaluation and diagnosis.
  • Closure After the tissue sample is collected, the incision is closed using sutures. Proper closure is essential to promote healing and minimize scarring.

3. Post-Procedure

Following the biopsy procedure coded as CPT® 27324, patients are typically monitored for any immediate complications, such as excessive bleeding or infection. Post-procedure care instructions may include keeping the biopsy site clean and dry, monitoring for signs of infection, and managing pain with prescribed medications. Patients are usually advised to avoid strenuous activities for a specified period to allow for proper healing. Follow-up appointments may be scheduled to discuss the histological results and any further management based on the findings.

Short Descr BIOPSY THIGH SOFT TISSUES
Medium Descr BIOPSY SOFT TISSUE THIGH/KNEE AREA DEEP
Long Descr Biopsy, soft tissue of thigh or knee area; deep (subfascial or intramuscular)
Status Code Active Code
Global Days 090 - Major Surgery
PC/TC Indicator (26, TC) 0 - Physician Service Code
Multiple Procedures (51) 2 - Standard payment adjustment rules for multiple procedures apply.
Bilateral Surgery (50) 1 - 150% payment adjustment for bilateral procedures applies.
Physician Supervisions 09 - Concept does not apply.
Assistant Surgeon (80, 82) 1 - Statutory payment restriction for assistants at surgery applies to this procedure...
Co-Surgeons (62) 0 - Co-surgeons not permitted for this procedure.
Team Surgery (66) 0 - Team surgeons not permitted for this procedure.
Diagnostic Imaging Family 99 - Concept Does Not Apply
APC Status Indicator Hospital Part B services paid through a comprehensive APC
ASC Payment Indicator Surgical procedure on ASC list in CY 2007; payment based on OPPS relative payment weight.
Type of Service (TOS) 2 - Surgery
Berenson-Eggers TOS (BETOS) P5B - Ambulatory procedures - musculoskeletal
MUE 3
CCS Clinical Classification 159 - Other diagnostic procedures on musculoskeletal system
59 Distinct procedural service: under certain circumstances, it may be necessary to indicate that a procedure or service was distinct or independent from other non-e/m services performed on the same day. modifier 59 is used to identify procedures/services, other than e/m services, that are not normally reported together, but are appropriate under the circumstances. documentation must support a different session, different procedure or surgery, different site or organ system, separate incision/excision, separate lesion, or separate injury (or area of injury in extensive injuries) not ordinarily encountered or performed on the same day by the same individual. however, when another already established modifier is appropriate it should be used rather than modifier 59. only if no more descriptive modifier is available, and the use of modifier 59 best explains the circumstances, should modifier 59 be used. note: modifier 59 should not be appended to an e/m service. to report a separate and distinct e/m service with a non-e/m service performed on the same date, see modifier 25.
AS Physician assistant, nurse practitioner, or clinical nurse specialist services for assistant at surgery
RT Right side (used to identify procedures performed on the right side of the body)
51 Multiple procedures: when multiple procedures, other than e/m services, physical medicine and rehabilitation services or provision of supplies (eg, vaccines), are performed at the same session by the same individual, the primary procedure or service may be reported as listed. the additional procedure(s) or service(s) may be identified by appending modifier 51 to the additional procedure or service code(s). note: this modifier should not be appended to designated "add-on" codes (see appendix d).
LT Left side (used to identify procedures performed on the left side of the body)
58 Staged or related procedure or service by the same physician or other qualified health care professional during the postoperative period: it may be necessary to indicate that the performance of a procedure or service during the postoperative period was: (a) planned or anticipated (staged); (b) more extensive than the original procedure; or (c) for therapy following a surgical procedure. this circumstance may be reported by adding modifier 58 to the staged or related procedure. note: for treatment of a problem that requires a return to the operating/procedure room (eg, unanticipated clinical condition), see modifier 78.
22 Increased procedural services: when the work required to provide a service is substantially greater than typically required, it may be identified by adding modifier 22 to the usual procedure code. documentation must support the substantial additional work and the reason for the additional work (ie, increased intensity, time, technical difficulty of procedure, severity of patient's condition, physical and mental effort required). note: this modifier should not be appended to an e/m service.
50 Bilateral procedure: unless otherwise identified in the listings, bilateral procedures that are performed at the same session, should be identified by adding modifier 50 to the appropriate 5 digit code. note: this modifier should not be appended to designated "add-on" codes (see appendix d).
62 Two surgeons: when 2 surgeons work together as primary surgeons performing distinct part(s) of a procedure, each surgeon should report his/her distinct operative work by adding modifier 62 to the procedure code and any associated add-on code(s) for that procedure as long as both surgeons continue to work together as primary surgeons. each surgeon should report the co-surgery once using the same procedure code. if additional procedure(s) (including add-on procedure(s) are performed during the same surgical session, separate code(s) may also be reported with modifier 62 added. note: if a co-surgeon acts as an assistant in the performance of additional procedure(s), other than those reported with the modifier 62, during the same surgical session, those services may be reported using separate procedure code(s) with modifier 80 or modifier 82 added, as appropriate.
76 Repeat procedure or service by same physician or other qualified health care professional: it may be necessary to indicate that a procedure or service was repeated by the same physician or other qualified health care professional subsequent to the original procedure or service. this circumstance may be reported by adding modifier 76 to the repeated procedure or service. note: this modifier should not be appended to an e/m service.
77 Repeat procedure by another physician or other qualified health care professional: it may be necessary to indicate that a basic procedure or service was repeated by another physician or other qualified health care professional subsequent to the original procedure or service. this circumstance may be reported by adding modifier 77 to the repeated procedure or service. note: this modifier should not be appended to an e/m service.
78 Unplanned return to the operating/procedure room by the same physician or other qualified health care professional following initial procedure for a related procedure during the postoperative period: it may be necessary to indicate that another procedure was performed during the postoperative period of the initial procedure (unplanned procedure following initial procedure). when this procedure is related to the first, and requires the use of an operating/procedure room, it may be reported by adding modifier 78 to the related procedure. (for repeat procedures, see modifier 76.)
79 Unrelated procedure or service by the same physician or other qualified health care professional during the postoperative period: the individual may need to indicate that the performance of a procedure or service during the postoperative period was unrelated to the original procedure. this circumstance may be reported by using modifier 79. (for repeat procedures on the same day, see modifier 76.)
80 Assistant surgeon: surgical assistant services may be identified by adding modifier 80 to the usual procedure number(s).
82 Assistant surgeon (when qualified resident surgeon not available): the unavailability of a qualified resident surgeon is a prerequisite for use of modifier 82 appended to the usual procedure code number(s).
CR Catastrophe/disaster related
GC This service has been performed in part by a resident under the direction of a teaching physician
GV Attending physician not employed or paid under arrangement by the patient's hospice provider
GW Service not related to the hospice patient's terminal condition
Q6 Service furnished under a fee-for-time compensation arrangement by a substitute physician or by a substitute physical therapist furnishing outpatient physical therapy services in a health professional shortage area, a medically underserved area, or a rural area
SG Ambulatory surgical center (asc) facility service
XS Separate structure, a service that is distinct because it was performed on a separate organ/structure
XU Unusual non-overlapping service, the use of a service that is distinct because it does not overlap usual components of the main service
Date
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2011-01-01 Changed Short description changed.
Pre-1990 Added Code added.
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