CPT® Code 85675

1. Indications

The thrombin time titer test (CPT® Code 85675) is indicated for various clinical scenarios where assessment of fibrinogen levels and functionality is necessary. The following conditions may warrant the performance of this test:

• Fibrinogen Deficiency This test is utilized to diagnose fibrinogen deficiency, which can be congenital or acquired.
• Congenital Conditions Specific congenital disorders such as afibrinogenemia, hypofibrinogenemia, and dysfibrinogenemia are indications for this test.
• Acquired Conditions Conditions such as liver disease, renal disease, disseminated intravascular coagulation (DIC), amyloidosis, malignancy, and the effects of anticoagulant drug therapy may also necessitate this test.

2. Procedure

The procedure for conducting the thrombin time titer test involves several critical steps to ensure accurate results. First, a blood sample is collected from the patient, typically via venipuncture. This sample is then processed to obtain plasma, which is the liquid component of blood that contains clotting factors. Next, the plasma is mixed with a standardized amount of human thrombin. This mixture is crucial as thrombin is the enzyme that catalyzes the conversion of fibrinogen to fibrin, leading to clot formation. The time taken for the clot to form is then measured using photometric methods, which assess the optical density of the sample as clotting occurs. This measurement provides the thrombin time titer, which reflects the functionality of fibrinogen in the sample. The results are then analyzed to determine if the clotting time falls within the normal range or indicates a deficiency or dysfunction of fibrinogen.

• Step 1: Blood Sample Collection A blood sample is collected from the patient through venipuncture to obtain plasma for testing.
• Step 2: Plasma Preparation The collected blood is processed to separate the plasma, which contains the necessary clotting factors.
• Step 3: Mixing with Thrombin The plasma is mixed with a standardized amount of human thrombin to initiate the clotting process.
• Step 4: Measuring Clotting Time The time taken for clot formation is measured photometrically, providing the thrombin time titer.

3. Post-Procedure

After the thrombin time titer test is completed, the patient may experience no significant post-procedure care requirements, as the test is minimally invasive. However, it is essential to monitor the patient for any immediate reactions to the blood draw. The results of the test will be analyzed and interpreted by the healthcare provider, who will discuss the findings with the patient and determine if further testing or treatment is necessary based on the thrombin time titer results. Follow-up may be required to address any underlying conditions indicated by the test results, particularly if a fibrinogen deficiency is diagnosed.