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Official Description

Digitization of glass microscope slides for immunofluorescence, per specimen; initial single antibody stain procedure (List separately in addition to code for primary procedure)

© Copyright 2025 American Medical Association. All rights reserved.

Common Language Description

The CPT® Code 0845T refers to the process of digitizing glass microscope slides specifically for immunofluorescence, focusing on the initial single antibody stain procedure. This procedure involves converting traditional glass microscope slides into a digital format, allowing for the captured images to be stored either on a local computer server or within a cloud-based archive. The digitization process enhances the ability to examine the slides remotely, enabling pathologists to analyze the images without the need for direct visualization through a microscope. This method not only facilitates remote consultations but also allows for the application of computer algorithms that can assist in providing pathologic diagnoses. The information obtained from the digitized slides can be managed and interpreted within an increasingly sophisticated image-based environment. The procedure is particularly relevant when performing an immunofluorescence antibody stain, such as on a skin biopsy specimen, where the digitization of the microscope slides occurs concurrently. It is important to report CPT® Code 0845T for the first initial single antibody stain, while subsequent single antibody stain digitizations should be reported using CPT® Code 0846T.

© Copyright 2025 Coding Ahead. All rights reserved.

1. Indications

The procedure associated with CPT® Code 0845T is indicated for the digitization of glass microscope slides in the context of immunofluorescence. This procedure is typically performed when there is a need to analyze tissue specimens, such as skin biopsies, using immunofluorescence techniques. The indications for this procedure may include, but are not limited to, the following:

  • Skin Biopsy Specimens The digitization is often performed on skin biopsy specimens to facilitate the examination of cellular and tissue structures using immunofluorescence.
  • Remote Pathology Consultations The need for remote analysis by pathologists who may not be physically present at the location of the specimen.
  • Enhanced Diagnostic Accuracy The use of computer algorithms to assist in the pathologic diagnosis, which may improve diagnostic accuracy compared to traditional methods.

2. Procedure

The procedure for CPT® Code 0845T involves several key steps that ensure the effective digitization of glass microscope slides for immunofluorescence. Each step is crucial for achieving high-quality digital images that can be utilized for further analysis.

  • Step 1: Preparation of the Specimen The initial step involves preparing the glass microscope slides that contain the tissue specimens. This may include ensuring that the specimens are properly stained with the appropriate immunofluorescence antibody for accurate visualization.
  • Step 2: Digitization Process Once the slides are prepared, they are placed into a digital scanner designed for microscope slides. The scanner captures high-resolution images of the slides, converting them into a digital format that can be stored and accessed electronically.
  • Step 3: Storage of Digital Images After digitization, the images are stored on a local computer server or within a cloud-based archive. This storage method allows for easy retrieval and sharing of the images among healthcare professionals.
  • Step 4: Remote Examination The digitized images can then be examined remotely by pathologists or other medical professionals. This step is essential for facilitating consultations and collaborative diagnoses, especially when specialists are not available on-site.
  • Step 5: Application of Computer Algorithms In some cases, computer algorithms may be employed to analyze the digitized images further. This technology can assist in identifying patterns or anomalies that may be indicative of specific pathologies.

3. Post-Procedure

Post-procedure care following the digitization of glass microscope slides for immunofluorescence primarily involves the management and interpretation of the digital images. Once the slides have been digitized and stored, healthcare professionals can access the images for review and diagnosis. It is important to ensure that the digital storage system is secure and that access is limited to authorized personnel to maintain patient confidentiality and data integrity. Additionally, any findings from the digital examination should be documented appropriately in the patient's medical record, and follow-up actions should be determined based on the results of the immunofluorescence analysis.

Short Descr DGTZ GLS MCRSCP IMFLUOR 1ST
Medium Descr DGTZ GLS MCRSCP SLD IMFLUOR 1ST SINGLE ANTB STN
Long Descr Digitization of glass microscope slides for immunofluorescence, per specimen; initial single antibody stain procedure (List separately in addition to code for primary procedure)
Status Code Carriers Price the Code
Global Days ZZZ - Code Related to Another Service
PC/TC Indicator (26, TC) 3 - Technical Component Only Code
Multiple Procedures (51) 0 - No payment adjustment rules for multiple procedures apply.
Bilateral Surgery (50) 0 - 150% payment adjustment for bilateral procedures does NOT apply.
Physician Supervisions 09 - Concept does not apply.
Assistant Surgeon (80, 82) 0 - Payment restriction for assistants at surgery applies to this procedure...
Co-Surgeons (62) 0 - Co-surgeons not permitted for this procedure.
Team Surgery (66) 0 - Team surgeons not permitted for this procedure.
Diagnostic Imaging Family 99 - Concept Does Not Apply
APC Status Indicator Items and Services Packaged into APC Rates
Berenson-Eggers TOS (BETOS) none
MUE Not applicable/unspecified.

This is an add-on code that must be used in conjunction with one of these primary codes.

88346 MPFS Status: Active Code APC Q2 PUB 100 CPT Assistant Article Immunofluorescence, per specimen; initial single antibody stain procedure
Date
Action
Notes
2024-01-01 Added Code Added.
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