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Morphometric analysis, in situ hybridization (quantitative or semi-quantitative) is a specialized laboratory technique utilized primarily by pathologists and geneticists to visualize and analyze specific genes or segments of genes within biological specimens. This method employs fluorescent tagging to detect genetic abnormalities, which may include translocations, deletions, duplications, amplifications, and inversions on chromosomes, as well as in hematologic cells and solid tumor cancers. The process begins with the fixation of cells onto a microscope slide, followed by treatment to denature the DNA, converting it into single strands. A short sequence of single-stranded DNA, known as a probe, is designed to match a specific portion of the target gene(s) and is tagged with fluorescent labels. This probe is then applied to the slide, where it hybridizes, or binds, to the complementary DNA strands present in the specimen. After allowing sufficient time for hybridization, the slide is washed to eliminate any unbound probe, ensuring that only the specifically hybridized areas remain. The final step involves manual examination of the slide under a microscope, where the pathologist or geneticist can identify and interpret the presence of genetic abnormalities. This detailed analysis is crucial for determining patient eligibility for targeted treatments, planning appropriate treatment courses, and monitoring the effectiveness of ongoing therapies. For billing purposes, CPT® Code 88368 is used for the initial single probe stain performed, while CPT® Code 88369 is designated for each additional single probe stain procedure conducted on the same specimen.
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The morphometric analysis, in situ hybridization (quantitative or semi-quantitative) procedure is indicated for the following conditions:
The procedure for morphometric analysis, in situ hybridization involves several key steps, each critical to ensuring accurate results:
Post-procedure care for morphometric analysis, in situ hybridization typically involves the careful documentation of findings and results. The pathologist or geneticist will compile the data obtained from the microscopic examination, which may include detailed notes on the presence and type of genetic abnormalities identified. This information is crucial for informing treatment decisions and may be shared with the patient's healthcare team. Additionally, any necessary follow-up procedures or further testing may be recommended based on the results of the analysis. It is important to ensure that all findings are accurately recorded and communicated to support ongoing patient care and management.
| Short Descr | M/PHMTRC ALYSISHQUANT/SEMIQ | Medium Descr | M/PHMTRC ALYS ISH QUANT/SEMIQ MNL PER SPEC EACH | Long Descr | Morphometric analysis, in situ hybridization (quantitative or semi-quantitative), manual, per specimen; each additional single probe stain procedure (List separately in addition to code for primary procedure) | Status Code | Active Code | Global Days | ZZZ - Code Related to Another Service | PC/TC Indicator (26, TC) | 1 - Diagnostic Tests for Radiology Services | Multiple Procedures (51) | 0 - No payment adjustment rules for multiple procedures apply. | Bilateral Surgery (50) | 0 - 150% payment adjustment for bilateral procedures does NOT apply. | Physician Supervisions | 09 - Concept does not apply. | Assistant Surgeon (80, 82) | 0 - Payment restriction for assistants at surgery applies to this procedure... | Co-Surgeons (62) | 0 - Co-surgeons not permitted for this procedure. | Team Surgery (66) | 0 - Team surgeons not permitted for this procedure. | Diagnostic Imaging Family | 99 - Concept Does Not Apply | APC Status Indicator | Items and Services Packaged into APC Rates | Type of Service (TOS) | 5 - Diagnostic Laboratory | Berenson-Eggers TOS (BETOS) | T1G - Lab tests - other (Medicare fee schedule) | MUE | 3 |
This is an add-on code that must be used in conjunction with one of these primary codes.
| 0852T | Add On Code Resequenced Code MPFS Status: Carrier Priced APC N Digitization of glass microscope slides for morphometric analysis, in situ hybridization (quantitative or semiquantitative), manual, per specimen; each additional single probe stain procedure (List separately in addition to code for primary procedure) | 88368 | MPFS Status: Active Code APC Q2 PUB 100 CPT Assistant Article Morphometric analysis, in situ hybridization (quantitative or semi-quantitative), manual, per specimen; initial single probe stain procedure | 0852T | Add On Code Resequenced Code MPFS Status: Carrier Priced APC N Digitization of glass microscope slides for morphometric analysis, in situ hybridization (quantitative or semiquantitative), manual, per specimen; each additional single probe stain procedure (List separately in addition to code for primary procedure) |
| 26 | Professional component: certain procedures are a combination of a physician or other qualified health care professional component and a technical component. when the physician or other qualified health care professional component is reported separately, the service may be identified by adding modifier 26 to the usual procedure number. | 59 | Distinct procedural service: under certain circumstances, it may be necessary to indicate that a procedure or service was distinct or independent from other non-e/m services performed on the same day. modifier 59 is used to identify procedures/services, other than e/m services, that are not normally reported together, but are appropriate under the circumstances. documentation must support a different session, different procedure or surgery, different site or organ system, separate incision/excision, separate lesion, or separate injury (or area of injury in extensive injuries) not ordinarily encountered or performed on the same day by the same individual. however, when another already established modifier is appropriate it should be used rather than modifier 59. only if no more descriptive modifier is available, and the use of modifier 59 best explains the circumstances, should modifier 59 be used. note: modifier 59 should not be appended to an e/m service. to report a separate and distinct e/m service with a non-e/m service performed on the same date, see modifier 25. | TC | Technical component; under certain circumstances, a charge may be made for the technical component alone; under those circumstances the technical component charge is identified by adding modifier 'tc' to the usual procedure number; technical component charges are institutional charges and not billed separately by physicians; however, portable x-ray suppliers only bill for technical component and should utilize modifier tc; the charge data from portable x-ray suppliers will then be used to build customary and prevailing profiles | GC | This service has been performed in part by a resident under the direction of a teaching physician | 76 | Repeat procedure or service by same physician or other qualified health care professional: it may be necessary to indicate that a procedure or service was repeated by the same physician or other qualified health care professional subsequent to the original procedure or service. this circumstance may be reported by adding modifier 76 to the repeated procedure or service. note: this modifier should not be appended to an e/m service. | XS | Separate structure, a service that is distinct because it was performed on a separate organ/structure | CR | Catastrophe/disaster related | Q1 | Routine clinical service provided in a clinical research study that is in an approved clinical research study | 91 | Repeat clinical diagnostic laboratory test: in the course of treatment of the patient, it may be necessary to repeat the same laboratory test on the same day to obtain subsequent (multiple) test results. under these circumstances, the laboratory test performed can be identified by its usual procedure number and the addition of modifier 91. note: this modifier may not be used when tests are rerun to confirm initial results; due to testing problems with specimens or equipment; or for any other reason when a normal, one-time, reportable result is all that is required. this modifier may not be used when other code(s) describe a series of test results (eg, glucose tolerance tests, evocative/suppression testing). this modifier may only be used for laboratory test(s) performed more than once on the same day on the same patient. | X5 | Diagnostic services requested by another clinician: for reporting services by a clinician who furnishes care to the patient only as requested by another clinician or subsequent and related services requested by another clinician; this modifier is reported for patient relationships that may not be adequately captured by the above alternative categories; reporting clinician service examples include but are not limited to, the radiologist's interpretation of an imaging study requested by another clinician | XU | Unusual non-overlapping service, the use of a service that is distinct because it does not overlap usual components of the main service | XE | Separate encounter, a service that is distinct because it occurred during a separate encounter | 77 | Repeat procedure by another physician or other qualified health care professional: it may be necessary to indicate that a basic procedure or service was repeated by another physician or other qualified health care professional subsequent to the original procedure or service. this circumstance may be reported by adding modifier 77 to the repeated procedure or service. note: this modifier should not be appended to an e/m service. | 90 | Reference (outside) laboratory: when laboratory procedures are performed by a party other than the treating or reporting physician or other qualified health care professional, the procedure may be identified by adding modifier 90 to the usual procedure number. | AQ | Physician providing a service in an unlisted health professional shortage area (hpsa) | GV | Attending physician not employed or paid under arrangement by the patient's hospice provider | GW | Service not related to the hospice patient's terminal condition | GZ | Item or service expected to be denied as not reasonable and necessary | Q6 | Service furnished under a fee-for-time compensation arrangement by a substitute physician or by a substitute physical therapist furnishing outpatient physical therapy services in a health professional shortage area, a medically underserved area, or a rural area | XP | Separate practitioner, a service that is distinct because it was performed by a different practitioner |
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| 2024-01-01 | Changed | Guideline added. |
| 2015-01-01 | Added | Added |
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