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Official Description

Digitization of glass microscope slides for in situ hybridization (eg, FISH), per specimen; each multiplex probe stain procedure (List separately in addition to code for primary procedure)

© Copyright 2025 American Medical Association. All rights reserved.

Common Language Description

The CPT® Code 0850T refers to the process of digitizing glass microscope slides specifically for in situ hybridization procedures, such as fluorescence in situ hybridization (FISH). This procedure involves converting traditional glass slides into a digital format, allowing for the captured images to be stored either on a local computer server or within a cloud-based archive. The digitization of these slides facilitates further examination by pathologists who may be located remotely, as well as the use of advanced computer algorithms that assist in providing pathologic diagnoses. This method enhances the ability to analyze tissue biopsy specimens stained for specific target RNA or DNA, as it allows for a more comprehensive interpretation of the results in an increasingly image-based diagnostic environment. It is important to note that this code is specifically for each multiplex probe stain procedure and should be reported separately in addition to the code for the primary procedure. For instance, when a single probe stain preparation is performed, the corresponding code 0848T should be reported, while code 0849T is designated for each additional single probe stain. The use of code 0850T is essential for accurately capturing the digitization of each multiplex probe stain, ensuring proper documentation and billing for these advanced diagnostic services.

© Copyright 2025 Coding Ahead. All rights reserved.

1. Indications

The digitization of glass microscope slides for in situ hybridization is indicated for the analysis of tissue biopsy specimens where the identification of specific target RNA or DNA is necessary. This procedure is particularly useful in cases where remote evaluation by pathologists is required or when advanced computational analysis is employed to enhance diagnostic accuracy. The use of multiplex probe stains allows for the simultaneous detection of multiple targets, making this procedure essential in complex diagnostic scenarios.

  • Tissue Biopsy Analysis Required for identifying specific target RNA/DNA in biopsy specimens.
  • Remote Pathologist Evaluation Facilitates examination by pathologists who may not be physically present.
  • Advanced Computational Analysis Utilizes computer algorithms to assist in pathologic diagnosis.
  • Multiplex Probe Staining Allows for the simultaneous detection of multiple genetic targets.

2. Procedure

The procedure for digitizing glass microscope slides for in situ hybridization involves several key steps that ensure accurate and effective results. First, a stained slide of a tissue biopsy specimen is prepared, which involves applying specific stains that target the RNA or DNA of interest. This preparation is crucial as it sets the foundation for the subsequent analysis. Next, the prepared slide is scanned using a specialized digital microscope that captures high-resolution images of the stained specimen. This scanning process converts the physical slide into a digital format, allowing for detailed examination and analysis. Once digitized, the images are stored securely on a local server or in a cloud-based archive, making them accessible for further review. The digital images can then be analyzed by pathologists remotely or processed using advanced algorithms to assist in diagnosing the specimen. Each multiplex probe stain procedure is reported separately using CPT® Code 0850T, ensuring that the complexity and detail of the analysis are accurately captured in the billing process.

  • Step 1: Slide Preparation A stained slide of a tissue biopsy specimen is prepared, applying specific stains to target RNA/DNA.
  • Step 2: Scanning The prepared slide is scanned using a digital microscope, converting it into a high-resolution digital format.
  • Step 3: Image Storage The digitized images are stored on a local server or cloud-based archive for accessibility.
  • Step 4: Analysis The digital images are analyzed by pathologists or processed with algorithms for diagnostic purposes.

3. Post-Procedure

After the digitization of glass microscope slides for in situ hybridization, the expected post-procedure care primarily involves the management and interpretation of the digital images. Pathologists may review the digitized slides remotely, allowing for timely diagnoses without the need for physical presence. Additionally, the digital format enables the use of advanced image analysis software, which can assist in identifying specific genetic markers or abnormalities. It is essential to ensure that the digital images are securely stored and backed up to prevent data loss. Furthermore, any findings from the analysis should be documented thoroughly, as this information is critical for patient management and follow-up care. There are no specific recovery considerations for the procedure itself, as it is a technical process rather than an invasive procedure.

Short Descr DGTZ GLS MCRSCP ISH EA MULT
Medium Descr DGTZ GLS MCRSCP SLD ISH EA MULT PROBE STAIN
Long Descr Digitization of glass microscope slides for in situ hybridization (eg, FISH), per specimen; each multiplex probe stain procedure (List separately in addition to code for primary procedure)
Status Code Carriers Price the Code
Global Days ZZZ - Code Related to Another Service
PC/TC Indicator (26, TC) 3 - Technical Component Only Code
Multiple Procedures (51) 0 - No payment adjustment rules for multiple procedures apply.
Bilateral Surgery (50) 0 - 150% payment adjustment for bilateral procedures does NOT apply.
Physician Supervisions 09 - Concept does not apply.
Assistant Surgeon (80, 82) 0 - Payment restriction for assistants at surgery applies to this procedure...
Co-Surgeons (62) 0 - Co-surgeons not permitted for this procedure.
Team Surgery (66) 0 - Team surgeons not permitted for this procedure.
Diagnostic Imaging Family 99 - Concept Does Not Apply
APC Status Indicator Items and Services Packaged into APC Rates
Berenson-Eggers TOS (BETOS) none
MUE Not applicable/unspecified.

This is an add-on code that must be used in conjunction with one of these primary codes.

88366 MPFS Status: Active Code APC Q1 In situ hybridization (eg, FISH), per specimen; each multiplex probe stain procedure
Date
Action
Notes
2024-01-01 Added Code Added.
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