CPT® Code 0853T

Add-On Code

1. Indications

The procedure associated with CPT® Code 0853T is indicated for the following conditions and scenarios:

• Pathological Examination The digitization of glass microscope slides is performed to facilitate detailed pathological examinations, allowing for enhanced analysis of tissue samples.
• Genetic Analysis This procedure is utilized in genetic studies where specific genes or gene portions need to be visualized, particularly in the context of in situ hybridization.
• Remote Consultation The digitization allows pathologists to review and analyze slides remotely, which is essential for consultations and collaborative diagnostics.
• Quantitative and Semi-Quantitative Analysis The procedure supports morphometric analysis, which can be quantitative or semi-quantitative, providing valuable data for research and clinical decision-making.

2. Procedure

The procedure for CPT® Code 0853T involves several key steps that ensure the effective digitization of glass microscope slides for morphometric analysis and in situ hybridization:

• Step 1: Preparation of Glass Slides Initially, glass slides containing tissue samples, such as those from bone marrow or tumors, are prepared using manual techniques. This preparation is crucial for ensuring that the samples are suitable for analysis and that the staining process can be effectively applied.
• Step 2: Application of Multiplex Probe Stains Following the preparation, multiplex probe stains are applied to the slides. This step is essential for visualizing multiple genetic targets simultaneously, which enhances the diagnostic capabilities of the analysis.
• Step 3: Digitization of Slides Once the staining is complete, the glass microscope slides are scanned to create digital images. This digitization process captures the details of the stained samples and converts them into a format that can be stored and analyzed digitally.
• Step 4: Storage and Management of Digital Images The digitized images are then stored on a local computer server or within a cloud-based archive. This storage solution allows for easy access and management of the images, facilitating further analysis and interpretation by pathologists or through computer algorithms.

3. Post-Procedure

After the digitization procedure is completed, the digital images of the glass microscope slides can be utilized for various purposes. Pathologists may review the images for diagnostic purposes, and the data can be analyzed using morphometric techniques. It is important to ensure that the digital files are securely stored and backed up to prevent data loss. Additionally, any findings or interpretations derived from the digitized images should be documented appropriately for future reference and compliance with medical record-keeping standards. The expected recovery from this procedure is immediate, as it primarily involves the digitization of existing slides rather than any invasive intervention.