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Official Description

Laparoscopy, surgical; with biopsy (single or multiple)

© Copyright 2025 American Medical Association. All rights reserved.

Common Language Description

The procedure described by CPT® Code 49321 refers to a laparoscopic surgical technique that involves the use of a laparoscope to perform a biopsy. This minimally invasive procedure begins with the placement of a periumbilical port, which is a small incision made near the belly button. Through this port, pneumoperitoneum is established, meaning that air is insufflated into the abdominal cavity to create a working space for the surgeon. The laparoscope, equipped with a video camera, is then inserted, allowing for a visual inspection of the entire abdominal cavity, including the peritoneum and omentum. This inspection is crucial for identifying any signs of malignancy, disease, or injury. During the procedure, biopsy forceps are introduced through the laparoscope to obtain tissue samples from one or multiple sites within the abdominal cavity. These samples are subsequently sent to a laboratory for histologic evaluation, which is reported separately. After the biopsy is completed, the surgeon inspects the biopsy sites for any bleeding, which is controlled using laser or electrocautery if necessary. Finally, the instruments are withdrawn, and pressure is applied to the abdomen to expel any remaining air from the peritoneum before closing the portal incisions. This procedure is essential for diagnosing various abdominal conditions while minimizing recovery time and complications associated with more invasive surgical techniques.

© Copyright 2025 Coding Ahead. All rights reserved.

1. Indications

The laparoscopic surgical procedure with biopsy, as described by CPT® Code 49321, is indicated for various clinical scenarios where tissue sampling is necessary to diagnose potential abnormalities within the abdominal cavity. The following conditions may warrant this procedure:

  • Suspicion of Malignancy - When there is a clinical suspicion of cancer based on imaging studies or physical examination findings.
  • Abdominal Pain - In cases of unexplained abdominal pain where further investigation is required to identify underlying causes.
  • Inflammatory Diseases - For conditions such as appendicitis or diverticulitis, where tissue evaluation may be necessary.
  • Infectious Processes - When there is a need to assess for infectious diseases affecting the abdominal organs.
  • Other Abnormal Findings - Any abnormal findings during imaging studies that require histological confirmation.

2. Procedure

The laparoscopic biopsy procedure involves several key steps that ensure effective tissue sampling while minimizing patient trauma. The following procedural steps are performed:

  • Step 1: Establishing Access - A periumbilical port is created by making a small incision near the belly button. This port serves as the entry point for the laparoscope and other instruments.
  • Step 2: Creating Pneumoperitoneum - Air is insufflated into the abdominal cavity through the port to create pneumoperitoneum, which expands the abdominal space and allows for better visualization and access to internal structures.
  • Step 3: Inserting the Laparoscope - The laparoscope, which is a thin tube with a camera, is inserted through the port. This device provides a visual feed to the surgical team, allowing them to inspect the abdominal cavity, peritoneum, and omentum for any abnormalities.
  • Step 4: Performing the Biopsy - Biopsy forceps are introduced through the laparoscope to obtain tissue samples. The surgeon may collect samples from one or multiple sites, depending on the findings during the inspection.
  • Step 5: Sending Samples for Evaluation - The collected tissue samples are sent to a laboratory for histologic evaluation, which is reported separately from the procedure itself.
  • Step 6: Inspecting for Bleeding - After the biopsy, the surgeon inspects the biopsy sites for any signs of bleeding. If bleeding is detected, it is controlled using laser or electrocautery techniques.
  • Step 7: Closing the Incisions - Once the procedure is complete, the instruments are withdrawn, and pressure is applied to the abdomen to expel any remaining air. The portal incisions are then closed to complete the procedure.

3. Post-Procedure

Following the laparoscopic biopsy procedure, patients are typically monitored for any immediate complications, such as bleeding or infection. Recovery may vary depending on the individual, but patients can generally expect a shorter recovery time compared to open surgical procedures. Post-procedure care may include pain management, instructions for activity restrictions, and follow-up appointments to discuss biopsy results. It is important for patients to report any unusual symptoms, such as increased pain, fever, or signs of infection, to their healthcare provider promptly. Overall, the laparoscopic approach allows for effective tissue sampling with minimal disruption to the patient's body, facilitating a quicker return to normal activities.

Short Descr LAPAROSCOPY BIOPSY
Medium Descr LAPAROSCOPY SURG W/BX SINGLE/MULTIPLE
Long Descr Laparoscopy, surgical; with biopsy (single or multiple)
Status Code Active Code
Global Days 010 - Minor Procedure
PC/TC Indicator (26, TC) 0 - Physician Service Code
Multiple Procedures (51) 3 - Special payment adjustment rules for multiple endoscopic procedures apply.
Bilateral Surgery (50) 0 - 150% payment adjustment for bilateral procedures does NOT apply.
Physician Supervisions 09 - Concept does not apply.
Assistant Surgeon (80, 82) 2 - Payment restriction for assistants at surgery does not apply to this procedure...
Co-Surgeons (62) 2 - Co-surgeons permitted and no documentation required if the two- specialty requirement is met.
Team Surgery (66) 0 - Team surgeons not permitted for this procedure.
Endoscopic Base Code 49320  Laparoscopy, abdomen, peritoneum, and omentum, diagnostic, with or without collection of specimen(s) by brushing or washing (separate procedure)
Diagnostic Imaging Family 99 - Concept Does Not Apply
APC Status Indicator Hospital Part B services paid through a comprehensive APC
ASC Payment Indicator Surgical procedure on ASC list in CY 2007; payment based on OPPS relative payment weight.
Type of Service (TOS) 2 - Surgery
Berenson-Eggers TOS (BETOS) P5E - Ambulatory procedures - other
MUE 1
CCS Clinical Classification 87 - Laparoscopy

This is a primary code that can be used with these additional add-on codes.

49327 Addon Code MPFS Status: Active Code APC N ASC N1 Laparoscopy, surgical; with placement of interstitial device(s) for radiation therapy guidance (eg, fiducial markers, dosimeter), intra-abdominal, intrapelvic, and/or retroperitoneum, including imaging guidance, if performed, single or multiple (List separately in addition to code for primary procedure)
AS Physician assistant, nurse practitioner, or clinical nurse specialist services for assistant at surgery
GC This service has been performed in part by a resident under the direction of a teaching physician
51 Multiple procedures: when multiple procedures, other than e/m services, physical medicine and rehabilitation services or provision of supplies (eg, vaccines), are performed at the same session by the same individual, the primary procedure or service may be reported as listed. the additional procedure(s) or service(s) may be identified by appending modifier 51 to the additional procedure or service code(s). note: this modifier should not be appended to designated "add-on" codes (see appendix d).
XS Separate structure, a service that is distinct because it was performed on a separate organ/structure
59 Distinct procedural service: under certain circumstances, it may be necessary to indicate that a procedure or service was distinct or independent from other non-e/m services performed on the same day. modifier 59 is used to identify procedures/services, other than e/m services, that are not normally reported together, but are appropriate under the circumstances. documentation must support a different session, different procedure or surgery, different site or organ system, separate incision/excision, separate lesion, or separate injury (or area of injury in extensive injuries) not ordinarily encountered or performed on the same day by the same individual. however, when another already established modifier is appropriate it should be used rather than modifier 59. only if no more descriptive modifier is available, and the use of modifier 59 best explains the circumstances, should modifier 59 be used. note: modifier 59 should not be appended to an e/m service. to report a separate and distinct e/m service with a non-e/m service performed on the same date, see modifier 25.
XU Unusual non-overlapping service, the use of a service that is distinct because it does not overlap usual components of the main service
82 Assistant surgeon (when qualified resident surgeon not available): the unavailability of a qualified resident surgeon is a prerequisite for use of modifier 82 appended to the usual procedure code number(s).
22 Increased procedural services: when the work required to provide a service is substantially greater than typically required, it may be identified by adding modifier 22 to the usual procedure code. documentation must support the substantial additional work and the reason for the additional work (ie, increased intensity, time, technical difficulty of procedure, severity of patient's condition, physical and mental effort required). note: this modifier should not be appended to an e/m service.
47 Anesthesia by surgeon: regional or general anesthesia provided by the surgeon may be reported by adding modifier 47 to the basic service. (this does not include local anesthesia.) note: modifier 47 would not be used as a modifier for the anesthesia procedures.
52 Reduced services: under certain circumstances a service or procedure is partially reduced or eliminated at the discretion of the physician or other qualified health care professional. under these circumstances the service provided can be identified by its usual procedure number and the addition of modifier 52, signifying that the service is reduced. this provides a means of reporting reduced services without disturbing the identification of the basic service. note: for hospital outpatient reporting of a previously scheduled procedure/service that is partially reduced or cancelled as a result of extenuating circumstances or those that threaten the well-being of the patient prior to or after administration of anesthesia, see modifiers 73 and 74 (see modifiers approved for asc hospital outpatient use).
53 Discontinued procedure: under certain circumstances, the physician or other qualified health care professional may elect to terminate a surgical or diagnostic procedure. due to extenuating circumstances or those that threaten the well being of the patient, it may be necessary to indicate that a surgical or diagnostic procedure was started but discontinued. this circumstance may be reported by adding modifier 53 to the code reported by the individual for the discontinued procedure. note: this modifier is not used to report the elective cancellation of a procedure prior to the patient's anesthesia induction and/or surgical preparation in the operating suite. for outpatient hospital/ambulatory surgery center (asc) reporting of a previously scheduled procedure/service that is partially reduced or cancelled as a result of extenuating circumstances or those that threaten the well being of the patient prior to or after administration of anesthesia, see modifiers 73 and 74 (see modifiers approved for asc hospital outpatient use).
58 Staged or related procedure or service by the same physician or other qualified health care professional during the postoperative period: it may be necessary to indicate that the performance of a procedure or service during the postoperative period was: (a) planned or anticipated (staged); (b) more extensive than the original procedure; or (c) for therapy following a surgical procedure. this circumstance may be reported by adding modifier 58 to the staged or related procedure. note: for treatment of a problem that requires a return to the operating/procedure room (eg, unanticipated clinical condition), see modifier 78.
62 Two surgeons: when 2 surgeons work together as primary surgeons performing distinct part(s) of a procedure, each surgeon should report his/her distinct operative work by adding modifier 62 to the procedure code and any associated add-on code(s) for that procedure as long as both surgeons continue to work together as primary surgeons. each surgeon should report the co-surgery once using the same procedure code. if additional procedure(s) (including add-on procedure(s) are performed during the same surgical session, separate code(s) may also be reported with modifier 62 added. note: if a co-surgeon acts as an assistant in the performance of additional procedure(s), other than those reported with the modifier 62, during the same surgical session, those services may be reported using separate procedure code(s) with modifier 80 or modifier 82 added, as appropriate.
78 Unplanned return to the operating/procedure room by the same physician or other qualified health care professional following initial procedure for a related procedure during the postoperative period: it may be necessary to indicate that another procedure was performed during the postoperative period of the initial procedure (unplanned procedure following initial procedure). when this procedure is related to the first, and requires the use of an operating/procedure room, it may be reported by adding modifier 78 to the related procedure. (for repeat procedures, see modifier 76.)
79 Unrelated procedure or service by the same physician or other qualified health care professional during the postoperative period: the individual may need to indicate that the performance of a procedure or service during the postoperative period was unrelated to the original procedure. this circumstance may be reported by using modifier 79. (for repeat procedures on the same day, see modifier 76.)
80 Assistant surgeon: surgical assistant services may be identified by adding modifier 80 to the usual procedure number(s).
81 Minimum assistant surgeon: minimum surgical assistant services are identified by adding modifier 81 to the usual procedure number.
AG Primary physician
AQ Physician providing a service in an unlisted health professional shortage area (hpsa)
CC Procedure code change (use 'cc' when the procedure code submitted was changed either for administrative reasons or because an incorrect code was filed)
CR Catastrophe/disaster related
GV Attending physician not employed or paid under arrangement by the patient's hospice provider
GW Service not related to the hospice patient's terminal condition
PD Diagnostic or related non diagnostic item or service provided in a wholly owned or operated entity to a patient who is admitted as an inpatient within 3 days
Q1 Routine clinical service provided in a clinical research study that is in an approved clinical research study
Q6 Service furnished under a fee-for-time compensation arrangement by a substitute physician or by a substitute physical therapist furnishing outpatient physical therapy services in a health professional shortage area, a medically underserved area, or a rural area
RT Right side (used to identify procedures performed on the right side of the body)
SG Ambulatory surgical center (asc) facility service
XE Separate encounter, a service that is distinct because it occurred during a separate encounter
XP Separate practitioner, a service that is distinct because it was performed by a different practitioner
Date
Action
Notes
2011-01-01 Changed Short description changed.
2001-01-01 Changed Code description changed.
2000-01-01 Added First appearance in code book in 2000.
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