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Chromatography is a sophisticated laboratory technique employed to separate and analyze chemical components within a mixture. This method utilizes two distinct phases: a mobile phase, which is typically a solvent, and a stationary phase, which is often a solid material such as silica gel or alumina. In the case of thin layer chromatography (TLC), the stationary phase is applied as a thin layer on a flat surface, such as a glass, metal, or plastic plate. The sample substance, referred to as the analyte, is introduced onto the stationary phase in the form of a dot or line. Once the sample is applied, the plate is placed in a container that contains a shallow layer of solvent. As the solvent ascends the stationary phase through capillary action, it carries the analyte along with it. The distance the analyte travels is influenced by its polarity, which affects its interaction with both the mobile and stationary phases. The qualitative aspect of this test indicates whether the analyte is present in the sample, but it does not provide quantitative measurements of the analyte's concentration. This technique is particularly useful in various fields, including chemistry and biochemistry, for identifying substances and assessing their presence in complex mixtures.
© Copyright 2025 Coding Ahead. All rights reserved.
The qualitative thin layer chromatography procedure, as described by CPT® Code 82489, is indicated for the analysis of various chemical substances when the specific analyte is not otherwise specified. This technique is particularly useful in situations where the presence of a particular compound needs to be confirmed without the necessity of quantifying its concentration. The following conditions may warrant the use of this procedure:
The thin layer chromatography procedure involves several key steps that ensure accurate separation and identification of the analyte. The following outlines the procedural steps:
After the thin layer chromatography procedure is completed, the results must be interpreted to determine the presence of the analyte. The chromatogram, which displays the separated components, is analyzed to identify the specific substances present in the sample. It is important to document the findings accurately, as this information may be critical for further testing or regulatory compliance. Additionally, any remaining samples and materials should be disposed of according to laboratory safety protocols to prevent contamination or exposure to hazardous substances.
Short Descr | THIN LAYER CHROMATOGRAPHY | Medium Descr | CHROMATOGRAPHY QUAL THIN LAYER ANALYTE NES | Long Descr | Chromatography, qualitative; thin layer, analyte not elsewhere specified | Status Code | Statutory Exclusion (from MPFS, may be paid under other methodologies) | Global Days | XXX - Global Concept Does Not Apply | PC/TC Indicator (26, TC) | 9 - Not Applicable | Multiple Procedures (51) | 9 - Concept does not apply. | Bilateral Surgery (50) | 9 - Concept does not apply. | Physician Supervisions | 09 - Concept does not apply. | Assistant Surgeon (80, 82) | 9 - Concept does not apply. | Co-Surgeons (62) | 9 - Concept does not apply. | Team Surgery (66) | 9 - Concept does not apply. | Diagnostic Imaging Family | 99 - Concept Does Not Apply | APC Status Indicator | Items and Services Packaged into APC Rates | Type of Service (TOS) | 5 - Diagnostic Laboratory | Berenson-Eggers TOS (BETOS) | T1H - Lab tests - other (non-Medicare fee schedule) | MUE | Not applicable/unspecified. | CCS Clinical Classification | 233 - Laboratory - Chemistry and Hematology |
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2016-01-01 | Deleted | Code deleted, to report see specific analyte code, or 84999 |
Pre-1990 | Added | Code added. |