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CPT®
82489

CPT® Code 82489
Deleted

Code deleted, to report see specific analyte code, or 84999

Official Description

Chromatography, qualitative; thin layer, analyte not elsewhere specified

© Copyright 2025 American Medical Association. All rights reserved.

Common Language Description

Chromatography is a sophisticated laboratory technique employed to separate and analyze chemical components within a mixture. This method utilizes two distinct phases: a mobile phase, which is typically a solvent, and a stationary phase, which is often a solid material such as silica gel or alumina. In the case of thin layer chromatography (TLC), the stationary phase is applied as a thin layer on a flat surface, such as a glass, metal, or plastic plate. The sample substance, referred to as the analyte, is introduced onto the stationary phase in the form of a dot or line. Once the sample is applied, the plate is placed in a container that contains a shallow layer of solvent. As the solvent ascends the stationary phase through capillary action, it carries the analyte along with it. The distance the analyte travels is influenced by its polarity, which affects its interaction with both the mobile and stationary phases. The qualitative aspect of this test indicates whether the analyte is present in the sample, but it does not provide quantitative measurements of the analyte's concentration. This technique is particularly useful in various fields, including chemistry and biochemistry, for identifying substances and assessing their presence in complex mixtures.

© Copyright 2025 Coding Ahead. All rights reserved.

1. Indications

The qualitative thin layer chromatography procedure, as described by CPT® Code 82489, is indicated for the analysis of various chemical substances when the specific analyte is not otherwise specified. This technique is particularly useful in situations where the presence of a particular compound needs to be confirmed without the necessity of quantifying its concentration. The following conditions may warrant the use of this procedure:

  • Substance Identification The procedure is utilized for identifying unknown substances in a sample, which may be critical in forensic analysis, environmental testing, or quality control in manufacturing.
  • Screening for Contaminants It is employed to screen for potential contaminants in food, pharmaceuticals, or biological samples, ensuring safety and compliance with regulatory standards.
  • Research Applications Researchers may use this technique to analyze chemical compounds in various studies, including drug development and biochemical research.

2. Procedure

The thin layer chromatography procedure involves several key steps that ensure accurate separation and identification of the analyte. The following outlines the procedural steps:

  • Step 1: Preparation of the Plate A thin layer of stationary phase, typically silica gel or alumina, is evenly spread on a glass, metal, or plastic plate. This layer must be uniform to ensure consistent results during the chromatography process.
  • Step 2: Application of the Sample A small dot or line of the sample substance (analyte) is carefully applied onto the stationary phase. This application must be precise to avoid overlapping with other samples and to ensure clear separation during the process.
  • Step 3: Development of the Chromatogram The plate is then placed in a container that contains a shallow layer of solvent. As the solvent moves up the plate through capillary action, it carries the analyte with it. The distance traveled by the analyte will depend on its polarity and interaction with both the stationary and mobile phases.
  • Step 4: Visualization After the solvent has traveled a sufficient distance, the plate is removed from the container and allowed to dry. Visualization techniques, such as UV light or staining, may be employed to make the separated analytes visible for analysis.

3. Post-Procedure

After the thin layer chromatography procedure is completed, the results must be interpreted to determine the presence of the analyte. The chromatogram, which displays the separated components, is analyzed to identify the specific substances present in the sample. It is important to document the findings accurately, as this information may be critical for further testing or regulatory compliance. Additionally, any remaining samples and materials should be disposed of according to laboratory safety protocols to prevent contamination or exposure to hazardous substances.
Short Descr THIN LAYER CHROMATOGRAPHY
Medium Descr CHROMATOGRAPHY QUAL THIN LAYER ANALYTE NES
Long Descr Chromatography, qualitative; thin layer, analyte not elsewhere specified
Status Code Statutory Exclusion (from MPFS, may be paid under other methodologies)
Global Days XXX - Global Concept Does Not Apply
PC/TC Indicator (26, TC) 9 - Not Applicable
Multiple Procedures (51) 9 - Concept does not apply.
Bilateral Surgery (50) 9 - Concept does not apply.
Physician Supervisions 09 - Concept does not apply.
Assistant Surgeon (80, 82) 9 - Concept does not apply.
Co-Surgeons (62) 9 - Concept does not apply.
Team Surgery (66) 9 - Concept does not apply.
Diagnostic Imaging Family 99 - Concept Does Not Apply
APC Status Indicator Items and Services Packaged into APC Rates
Type of Service (TOS) 5 - Diagnostic Laboratory
Berenson-Eggers TOS (BETOS) T1H - Lab tests - other (non-Medicare fee schedule)
MUE Not applicable/unspecified.
CCS Clinical Classification 233 - Laboratory - Chemistry and Hematology
Date
Action
Notes
2016-01-01 Deleted Code deleted, to report see specific analyte code, or 84999
Pre-1990 Added Code added.