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CPT®
82491

CPT® Code 82491
Deleted

Code deleted, to report see specific analyte code, or 82542

Official Description

Chromatography, quantitative, column (eg, gas liquid or HPLC); single analyte not elsewhere specified, single stationary and mobile phase

© Copyright 2025 American Medical Association. All rights reserved.

Common Language Description

Chromatography is a sophisticated laboratory technique utilized for the separation of chemical components within a mixture. This process employs two distinct phases: a stationary phase and a mobile phase. In the context of column chromatography, the stationary phase is established by applying a thin layer of a liquid or polymer that contains the test sample onto a solid, inert support material. This support is then inserted into a column, which can be constructed from glass or metal, and is sealed to maintain the integrity of the sample during analysis. To enhance the efficiency of the separation process, the column may be subjected to controlled heating using an oven or similar device, which regulates the temperature throughout the procedure. The mobile phase, which can be either a gas or a liquid solvent, is introduced into the system, initiating the separation of the sample's components, known as analytes. The movement of these analytes through the column is influenced by their polarity, which determines the distance they travel in conjunction with the solvent. The CPT® Code 82491 is specifically designated for the quantitative analysis of a single analyte that is not specified elsewhere. This code is essential for accurately measuring the concentration of various substances within a sample, such as Coenzyme Q10, thiopurine metabolites, iodide, citalopram, strychnine, lipid peroxides (TBARS), cyclobenzaprine (Flexeril), buspirone (Buspar), pyridostigmine (Mestinon), bendiocarb, chloral hydrate, and phenazopyridine (Phenazo). In contrast, CPT® Code 82492 is utilized for the quantification of multiple analytes within a single sample, including substances like parathion, ketamine, and fractionated porphyrins.

© Copyright 2025 Coding Ahead. All rights reserved.

1. Indications

The procedure associated with CPT® Code 82491 is indicated for the quantitative analysis of specific chemical substances within a sample. This analysis is crucial for various clinical and research applications where precise measurement of a single analyte is required. The following conditions or scenarios may warrant the use of this procedure:

  • Measurement of Coenzyme Q10 for assessing mitochondrial function or antioxidant status.
  • Quantification of thiopurine metabolites to monitor therapeutic levels in patients undergoing treatment for autoimmune diseases or certain cancers.
  • Analysis of iodide levels for evaluating thyroid function or dietary intake.
  • Assessment of citalopram concentrations in patients receiving treatment for depression to ensure appropriate dosing.
  • Detection of strychnine in toxicology screenings to identify potential poisoning cases.
  • Measurement of lipid peroxides (TBARS) as a marker for oxidative stress in various health conditions.
  • Quantification of cyclobenzaprine (Flexeril) to monitor levels in patients prescribed this muscle relaxant.
  • Assessment of buspirone (Buspar) levels for patients being treated for anxiety disorders.
  • Measurement of pyridostigmine (Mestinon) for monitoring treatment in myasthenia gravis patients.
  • Detection of bendiocarb in toxicology tests to evaluate exposure to this pesticide.
  • Analysis of chloral hydrate levels in patients receiving this sedative for sleep disorders.
  • Quantification of phenazopyridine (Phenazo) to assess levels in patients using this medication for urinary tract discomfort.

2. Procedure

The procedure for CPT® Code 82491 involves several critical steps to ensure accurate quantification of the specified analyte. The process begins with the preparation of the sample, where a microscopic layer of the liquid or polymer containing the analyte is applied to a solid, inert support. This support is then placed into a column, which is typically made of glass or metal, and sealed to prevent contamination or loss of the sample. The column is often subjected to controlled heating, which helps to optimize the separation process by regulating the temperature of the mobile phase.

  • Step 1: Sample Preparation - The test sample is prepared by applying it to the stationary phase on the solid support, ensuring that it is evenly distributed for optimal separation.
  • Step 2: Column Setup - The prepared support is inserted into the column, which is then sealed to maintain the integrity of the sample during the chromatography process.
  • Step 3: Temperature Control - The column may be placed in an oven or similar device to control the temperature, which is crucial for the efficiency of the mobile phase.
  • Step 4: Mobile Phase Introduction - The mobile phase, either a gas or liquid solvent, is introduced into the column, initiating the separation of the analytes based on their polarity.
  • Step 5: Separation and Quantification - As the mobile phase moves through the column, the analytes separate from one another, allowing for the quantification of the single analyte of interest.

3. Post-Procedure

After the chromatography procedure is completed, the results are analyzed to determine the concentration of the single analyte in the sample. It is essential to document the findings accurately, as this data may be used for further clinical decision-making or research purposes. Depending on the specific analyte tested, additional follow-up may be required to interpret the results in the context of the patient's health or treatment plan. Proper storage and handling of the results are also crucial to maintain their integrity for future reference or audits.
Short Descr CHROMOTOGRAPHY QUANT SING
Medium Descr CHROMATOGRAPHY QUAN COLUMN 1 ANALYTE NES
Long Descr Chromatography, quantitative, column (eg, gas liquid or HPLC); single analyte not elsewhere specified, single stationary and mobile phase
Status Code Statutory Exclusion (from MPFS, may be paid under other methodologies)
Global Days XXX - Global Concept Does Not Apply
PC/TC Indicator (26, TC) 9 - Not Applicable
Multiple Procedures (51) 9 - Concept does not apply.
Bilateral Surgery (50) 9 - Concept does not apply.
Physician Supervisions 09 - Concept does not apply.
Assistant Surgeon (80, 82) 9 - Concept does not apply.
Co-Surgeons (62) 9 - Concept does not apply.
Team Surgery (66) 9 - Concept does not apply.
Diagnostic Imaging Family 99 - Concept Does Not Apply
APC Status Indicator Items and Services Packaged into APC Rates
Type of Service (TOS) 5 - Diagnostic Laboratory
Berenson-Eggers TOS (BETOS) T1H - Lab tests - other (non-Medicare fee schedule)
MUE Not applicable/unspecified.
CCS Clinical Classification 233 - Laboratory - Chemistry and Hematology
Date
Action
Notes
2016-01-01 Deleted Code deleted, to report see specific analyte code, or 82542
2011-01-01 Changed Short description changed.
1993-01-01 Added First appearance in code book in 1993.
Code
Description