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Official Description

Hemolysin, acid

© Copyright 2025 American Medical Association. All rights reserved.

Common Language Description

The CPT® Code 85475 refers to a laboratory test known as the Hemolysin, acid test, commonly referred to as the Ham test. This test is specifically designed to evaluate the condition and fragility of red blood cells (RBCs) when exposed to a mild acid solution. The procedure involves the addition of this acid to a blood sample, which allows healthcare professionals to observe how the RBCs respond, particularly in terms of their integrity and stability. The Ham test is particularly significant in diagnosing various blood disorders, both acquired and inherited. One notable condition that may be diagnosed through this test is dyserythropoietic anemia type II. This specific type of anemia is characterized by ineffective erythropoiesis, which leads to anemia and is associated with distinctive morphological abnormalities in the erythrocytes found in the bone marrow. To perform the test, a blood sample is collected, typically through a separately reportable venipuncture, ensuring that the sample is suitable for analysis. Both serum and whole blood are subjected to testing, which utilizes complement lysis techniques alongside visual identification methods to assess the fragility of the red blood cells accurately.

© Copyright 2025 Coding Ahead. All rights reserved.

1. Indications

The Ham test (CPT® Code 85475) is indicated for the evaluation of conditions related to the fragility of red blood cells (RBCs) and is particularly useful in diagnosing specific blood disorders. The following are the primary indications for performing this test:

  • Acquired Blood Disorders The test may be utilized to identify various acquired blood disorders that affect red blood cell stability and function.
  • Inherited Blood Disorders The Ham test is also applicable in diagnosing inherited conditions that impact red blood cell integrity, such as dyserythropoietic anemia type II.
  • Dyserythropoietic Anemia Type II This specific type of anemia is characterized by ineffective erythropoiesis, leading to anemia and distinctive morphological abnormalities in bone marrow erythrocytes.

2. Procedure

The procedure for the Ham test involves several critical steps to ensure accurate results. The following outlines the procedural steps involved:

  • Step 1: Blood Sample Collection A blood sample is obtained from the patient through a venipuncture, which is a separately reportable procedure. This step is crucial as it ensures that a suitable sample is collected for testing.
  • Step 2: Preparation of the Sample Once the blood sample is collected, it is prepared for testing. This involves separating the serum and whole blood components, which are necessary for the subsequent analysis.
  • Step 3: Addition of Mild Acid Solution A mild acid solution is then added to the blood sample. This step is essential as it allows for the observation of the red blood cells' fragility and response to the acidic environment.
  • Step 4: Testing for Complement Lysis The sample undergoes testing using complement lysis techniques. This method helps to assess the stability of the red blood cells when exposed to the acid.
  • Step 5: Visual Identification Finally, visual identification is performed to evaluate the condition of the red blood cells. This step is critical for determining the presence of any abnormalities or fragility in the RBCs.

3. Post-Procedure

After the Ham test is completed, there are no specific post-procedure care requirements mentioned. However, it is essential for healthcare professionals to monitor the patient for any potential reactions to the blood draw. The results of the test will be analyzed and interpreted by the healthcare provider, who will then discuss the findings with the patient and determine any necessary follow-up actions based on the results.

Short Descr HEMOLYSIN ACID
Medium Descr HEMOLYSIN ACID
Long Descr Hemolysin, acid
Status Code Statutory Exclusion (from MPFS, may be paid under other methodologies)
Global Days XXX - Global Concept Does Not Apply
PC/TC Indicator (26, TC) 9 - Not Applicable
Multiple Procedures (51) 9 - Concept does not apply.
Bilateral Surgery (50) 9 - Concept does not apply.
Physician Supervisions 09 - Concept does not apply.
Assistant Surgeon (80, 82) 9 - Concept does not apply.
Co-Surgeons (62) 9 - Concept does not apply.
Team Surgery (66) 9 - Concept does not apply.
Diagnostic Imaging Family 99 - Concept Does Not Apply
CLIA Waived (QW) No
APC Status Indicator Conditionally packaged laboratory tests
Type of Service (TOS) 5 - Diagnostic Laboratory
Berenson-Eggers TOS (BETOS) T1H - Lab tests - other (non-Medicare fee schedule)
MUE 1
CCS Clinical Classification 233 - Laboratory - Chemistry and Hematology
90 Reference (outside) laboratory: when laboratory procedures are performed by a party other than the treating or reporting physician or other qualified health care professional, the procedure may be identified by adding modifier 90 to the usual procedure number.
Date
Action
Notes
2013-01-01 Changed Short Descriptor changed.
1993-01-01 Added First appearance in code book in 1993.
Code
Description
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