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Official Description

Reptilase test

© Copyright 2025 American Medical Association. All rights reserved.

Common Language Description

The Reptilase test, identified by CPT® Code 85635, is a specialized laboratory procedure utilized to assess the clotting time of blood. This test is particularly significant in the context of coagulation studies, as it serves as a follow-up to the thrombin time test. The primary purpose of the Reptilase test is to determine whether heparin is influencing the clotting process, which can lead to prolonged clotting times. In cases where heparin is present, the Reptilase test typically yields normal results, indicating that the clotting mechanism is functioning properly despite the presence of heparin. Conversely, if the results show a prolonged clotting time, this may suggest underlying issues such as a deficiency in fibrinogen, abnormalities in fibrinogen due to liver disease, or the presence of lupus anticoagulants in the plasma. The procedure involves the addition of reptilase to citrated blood plasma, followed by the measurement of the rate at which fibrin clots form, providing critical information for diagnosing and managing coagulation disorders.

© Copyright 2025 Coding Ahead. All rights reserved.

1. Indications

The Reptilase test is indicated for several specific clinical scenarios, particularly in the evaluation of coagulation disorders. The following conditions may warrant the performance of this test:

  • Follow-up to Thrombin Time Test The Reptilase test is often performed as a subsequent assessment after a thrombin time test to further investigate prolonged clotting times.
  • Assessment of Heparin Influence It is utilized to confirm the presence of heparin as a potential cause of prolonged clotting time, helping to differentiate between heparin-related effects and other coagulation abnormalities.
  • Evaluation of Fibrinogen Levels The test may be indicated when there is a suspicion of fibrinogen deficiency or abnormalities, which can be critical in diagnosing conditions related to liver disease.
  • Detection of Lupus Anticoagulants The Reptilase test can assist in identifying the presence of lupus anticoagulants in plasma, which are associated with increased risk of thrombosis.

2. Procedure

The Reptilase test involves a series of precise procedural steps to ensure accurate measurement of clotting time. The following outlines the key steps involved in the procedure:

  • Step 1: Sample Collection A blood sample is collected from the patient, typically using a venipuncture technique. The blood is then placed into a tube containing citrate, which acts as an anticoagulant to prevent clotting prior to testing.
  • Step 2: Preparation of Plasma The collected blood sample is centrifuged to separate the plasma from the cellular components. The resulting citrated plasma is then carefully extracted for testing.
  • Step 3: Addition of Reptilase Reptilase, an enzyme derived from the venom of the Bothrops atrox snake, is added to the citrated plasma. This enzyme facilitates the conversion of fibrinogen to fibrin, initiating the clotting process.
  • Step 4: Measurement of Clotting Time The rate of fibrin clot formation is monitored and measured. The time taken for the clot to form is recorded, which constitutes the result of the Reptilase test.

3. Post-Procedure

After the Reptilase test is completed, the results are analyzed and interpreted by a qualified healthcare professional. The expected recovery from the procedure is immediate, as it is a laboratory test that does not require any invasive intervention. Patients may resume normal activities following the blood draw. It is important for healthcare providers to communicate the results to the patient and discuss any necessary follow-up actions based on the findings, particularly if abnormalities are detected that may indicate underlying coagulation disorders.

Short Descr REPTILASE TEST
Medium Descr REPTILASE TEST
Long Descr Reptilase test
Status Code Statutory Exclusion (from MPFS, may be paid under other methodologies)
Global Days XXX - Global Concept Does Not Apply
PC/TC Indicator (26, TC) 9 - Not Applicable
Multiple Procedures (51) 9 - Concept does not apply.
Bilateral Surgery (50) 9 - Concept does not apply.
Physician Supervisions 09 - Concept does not apply.
Assistant Surgeon (80, 82) 9 - Concept does not apply.
Co-Surgeons (62) 9 - Concept does not apply.
Team Surgery (66) 9 - Concept does not apply.
Diagnostic Imaging Family 99 - Concept Does Not Apply
CLIA Waived (QW) No
APC Status Indicator Conditionally packaged laboratory tests
Type of Service (TOS) 5 - Diagnostic Laboratory
Berenson-Eggers TOS (BETOS) T1H - Lab tests - other (non-Medicare fee schedule)
MUE 1
CCS Clinical Classification 233 - Laboratory - Chemistry and Hematology
90 Reference (outside) laboratory: when laboratory procedures are performed by a party other than the treating or reporting physician or other qualified health care professional, the procedure may be identified by adding modifier 90 to the usual procedure number.
91 Repeat clinical diagnostic laboratory test: in the course of treatment of the patient, it may be necessary to repeat the same laboratory test on the same day to obtain subsequent (multiple) test results. under these circumstances, the laboratory test performed can be identified by its usual procedure number and the addition of modifier 91. note: this modifier may not be used when tests are rerun to confirm initial results; due to testing problems with specimens or equipment; or for any other reason when a normal, one-time, reportable result is all that is required. this modifier may not be used when other code(s) describe a series of test results (eg, glucose tolerance tests, evocative/suppression testing). this modifier may only be used for laboratory test(s) performed more than once on the same day on the same patient.
GA Waiver of liability statement issued as required by payer policy, individual case
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