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Official Description

Mechanical fragility, RBC

© Copyright 2025 American Medical Association. All rights reserved.

Common Language Description

The CPT® Code 85547 refers to a laboratory test that evaluates the mechanical fragility of red blood cells (RBCs). This test is crucial for understanding how susceptible RBCs are to hemolysis, which is the process of rupture or destruction of these cells when they are subjected to various forms of stress. Mechanical fragility testing is particularly challenging to standardize due to the complexities involved in simulating the shear stress that RBCs may encounter in clinical settings. Typically, the procedure involves placing a sample of whole blood into a test tube that contains glass beads. The test tube is then vigorously agitated, which mimics the mechanical forces that RBCs might experience during medical procedures. Following this agitation, the sample is analyzed for hemoglobin levels to assess the degree of damage sustained by the RBCs. This test is especially significant in contexts such as dialysis, intra-operative auto-transfusion, and other medical interventions that utilize devices like pumps, valves, cannulas, or gas exchange systems. Additionally, it is important to note that stored blood products can also undergo mechanical stress, leading to potential damage to the RBCs, making this test relevant for ensuring the quality and safety of transfused blood products.

© Copyright 2025 Coding Ahead. All rights reserved.

1. Indications

The mechanical fragility test of red blood cells (RBCs) is indicated in various clinical scenarios where the integrity of RBCs may be compromised. The following conditions and situations warrant the performance of this test:

  • Assessment during Dialysis This test is performed to evaluate the fragility of RBCs in patients undergoing dialysis, where mechanical forces can lead to cell damage.
  • Intra-operative Auto-transfusion The procedure is indicated for patients receiving intra-operative auto-transfusion, as the mechanical handling of blood can affect RBC integrity.
  • Use of Pumps and Valves It is relevant in situations involving the use of pumps, valves, and cannulas, which may exert mechanical stress on RBCs.
  • Gas Exchange Devices The test is indicated for patients undergoing procedures that utilize gas exchange devices, where RBCs may be subjected to shear stress.
  • Evaluation of Stored Blood Products This test is also indicated for assessing the mechanical fragility of stored blood products, which can be affected by storage conditions and handling.

2. Procedure

The procedure for testing mechanical fragility of red blood cells involves several key steps that ensure accurate assessment of RBC integrity. The following outlines the procedural steps:

  • Step 1: Sample Collection A sample of whole blood is collected from the patient using standard venipuncture techniques. It is essential to handle the blood sample carefully to prevent any pre-analytical factors that could affect the results.
  • Step 2: Preparation of Test Tube The collected blood sample is placed into a test tube that contains glass beads. These beads are crucial as they provide the necessary mechanical agitation during the test.
  • Step 3: Agitation The test tube is vigorously agitated to simulate the mechanical stress that RBCs may encounter in various clinical situations. This agitation is performed for a specified duration to ensure consistent results.
  • Step 4: Hemoglobin Measurement After agitation, the sample is analyzed for hemoglobin levels. This measurement is critical as it indicates the extent of hemolysis that has occurred as a result of the mechanical stress applied to the RBCs.
  • Step 5: Interpretation of Results The results are interpreted by comparing the hemoglobin levels before and after the mechanical stress. A significant decrease in hemoglobin indicates increased fragility of the RBCs.

3. Post-Procedure

Post-procedure care for the mechanical fragility test is generally minimal, as it is a laboratory-based test. However, it is important to monitor the patient for any adverse reactions related to the blood draw, such as bruising or discomfort at the venipuncture site. The results of the test should be communicated to the healthcare provider for further evaluation and management, especially if significant RBC fragility is detected. This information can be critical in guiding treatment decisions, particularly in patients undergoing procedures that may impact RBC integrity.

Short Descr RBC MECHANICAL FRAGILITY
Medium Descr MECHANICAL FRAGILITY RBC
Long Descr Mechanical fragility, RBC
Status Code Statutory Exclusion (from MPFS, may be paid under other methodologies)
Global Days XXX - Global Concept Does Not Apply
PC/TC Indicator (26, TC) 9 - Not Applicable
Multiple Procedures (51) 9 - Concept does not apply.
Bilateral Surgery (50) 9 - Concept does not apply.
Physician Supervisions 09 - Concept does not apply.
Assistant Surgeon (80, 82) 9 - Concept does not apply.
Co-Surgeons (62) 9 - Concept does not apply.
Team Surgery (66) 9 - Concept does not apply.
Diagnostic Imaging Family 99 - Concept Does Not Apply
CLIA Waived (QW) No
APC Status Indicator Conditionally packaged laboratory tests
Type of Service (TOS) 5 - Diagnostic Laboratory
Berenson-Eggers TOS (BETOS) T1H - Lab tests - other (non-Medicare fee schedule)
MUE 1
CCS Clinical Classification 233 - Laboratory - Chemistry and Hematology
Date
Action
Notes
Pre-1990 Added Code added.
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